Methyl pyropheophorbide-a

Research use only, not for human use.

Methyl pyropheophorbide-a (MPPa) is a chlorine photosensitizer and a derivative of chlorophyll a. It is photodynamically active, induces apoptosis and inhibits tumor growth, and can be used in photodynamic therapy (PDT) of cancer.

Methyl pyropheophorbide-a (Pyropheophorbide-a methyl ester), a chlorophyll-a derivative, is a potent photosensitizer that can be used in photodynamic therapy (PDT) of cancer. Methyl pyropheophorbide-a has photodynamic activity and can induce apoptosis and inhibit tumor growth.

Pyropheophorbide-a methyl ester (2 μM; 12 h) inhibits the cell cycle progression from the G0/G1-phases and induces apoptosis at the light dosage of 55.6 kJ/m2 in PC-3M cells.Pyropheophorbide-a methyl ester (0.2-15 μM; 20 h) has no signi?cant dark cytotoxicity in NCI-h446 cells.Pyropheophorbide-a methyl ester (0.1-15 μM; 20 h) mediates a dose-dependent photocytotoxicity in the NCI-h446 cells.Pyropheophorbide-a methyl ester (2-4 μM; 0-24 h) induces apoptosis of NCI-h446 cells in a time-dependent manner.Pyropheophorbide-a methyl ester (0.5-4 μM; 0-20 h) shows a photocytotoxicity in PC-3M cells.Apoptosis Analysis Cell Line:PC-3M cells Concentration:2 μM Incubation Time:12 h Result:Apoptosis rate was about 0.27%, 6.15%, 20.49%, 50.76%, 65.39%, 64.92%, and 64.37% at 1, 3, 6, 12, 18, and 24 h posttreatment, respectively under the conditions of LC75 (2 μM+55.6 kJ/m2).

Pyropheophorbide-a methyl ester (15 mg/kg; tail vein and topical injection) inhibits the growth of PC-3M tumors in mice.Animal Model:Male BALB/c nude mice (6-8 weeks, 16-20 g) bearing PC-3M tumor Dosage:15 mg/kg Administration:Tail vein injection and topical injection Result:The tumor volume and the weight inhibition rate were 46.78%, 41.84% in tail vein injection group and 78.66%, 72.07% in topical injection group, respectively.

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Apoptosis

Apoptosis

Apoptosis

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6453-67-4

548.67

C34H36N4O3

>98% (HPLC)

In Vitro:?DMSO : 2.5 mg/mL (4.56 mM; Ultrasonic (<60°C)

CCc1c(C)c2cc3[nH]c(cc4nc([C@@H](CCC(=O)OC)[C@@H]4C)c4CC(=O)c5c(C)c(cc1n2)[nH]c45)c(C)c3C=C

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(-20℃)

With Ice Pack

≥ 2 years